kmk	Services	Amount	Price
Non-viral services	Plasmid Midi-Prep	100 µg	$250.00
	Plasmid Maxi-Prep	500 µg	$450.00
	Transformation	1 plasmid	$50.00
	Plasmid cloning (1-2 steps)	xxx	$650.00
	Plasmid cloning (multi step)	Contact Core Manager(2)	xxx
	Aliquots of stock plasmid vector (3)	5 µg	$100.00
	Restriction Mapping	xxx	$100.00
xxx
Viral Services	Lentiviral vector construction	xxx	$750.00
	Aliquots of stock lentiviral vector (3)	5 µg	$100.00
	Lentiviral production-small scale Transfection and harvest of 30ml supernate from 150cm2 flask	30 ml (106 particles/ml)(4)	$300.00
	Lentiviral production-small scale(concentrated) Concentrate from 30 ml supernate	100 µl (109 particles/ml)(4)	$400.00
	Lentiviral production-large scale(concentrated) Concentrate from 150 ml supernate (five 150 cm2 flasks)	500 µl (109 particles/ml)(4)	$1,500.00
	Lentivirus-concentrated stock aliquots (3) click here for available stocks	50 µl (109particles/ml)(4)	$250.00
	Titration (fluorescent protein -expressing vectors)	1 vector	$100.00
	Titration (non-fluorescent protein-expressing vectors) Detection of vector or packaging sequences by PCR	1 vector	$250.00
	RCA by serial transfer and P24 ELISA	1 vector	$500.00
	RCA by serial transfer and P24 ELISA	3 vectors	$1,000.00
	P24 ELISA	1-10 samples	$350.00
	P24 ELISA	11-20 samples	$550.00
	P24 ELISA	21-35 samples	$700.00
	lenti-polymerase II knockdown construct (miRNA)	1 construct	$750.00
	lenti-polymerase II knockdown construct (miRNA)	3 constructs	$1,500.00
	lenti-polymerase III knockdown construct (shRNA)	1 construct	$750.00
	lenti-polymerase III knockdown construct (shRNA)	3 constructs	$1,500.00
kkk
Transgenesis	DNA purification for microinjection	xxx	$200.00
	Pronuclear injection for generating transgenic mice	2 founders	$3,000.00
	Zona lentiviral Injection for generating transgenic mice	3 founders	$2,000.00
	Pronuclear injection for generating transgenic rats	2 founders	$4,000.00
	Zona lentiviral Injection for generating transgenic rats	3 founders	$2,500.00
	Genotyping by PCR	per sample	$20.00
kkk
Knockout	Gene targeting construct	1 vector	$3,000.00
	ES cell targeting ES cell electroporation, clone picking, cell freezing, & DNA extraction	350 clones	$3,000.00
	ES cell expansion Expand and freeze selected clones, extract DNA for further analysis	2 transfers	$400.00
	Southern blot for screening including gDNA Up to 20 samples by one restriction enzyme, one probe hybridization	xxx	$1,000.00
	Blastocyst injection/embryo transfer	2 transfers	$1,500.00
kkk
Animal Bioassays	Teratoma analysis (Scid mice)	3 mice, 6 injections	$600.00
	Chimera analysis (mice)	2 transfers	$1,000.00
	Embryo transfer (mice)	1 transfer	$400.00
	Embryo transfer (rats)	1 transfer	$500.00
	Rederivation/Embryo transfer	Contact Core Manager(2)	xxx

(1) Rates apply to MWRI Investigators. External Investigators will be subject to an additional charge to defray indirect costs.
(2) Contact Core Manager
(3) For list of available plasmid vector, lentiviral vector, or lentivirus-constructed stocks, click here
(4) Titers can be variable and construct dependent.