Cliff J. Luke, Ph.D.
Assistant Investigator, Magee-Womens Research Institute
Assistant Professor, Pediatrics, University of Pittsburgh
Ph.D., University of Surrey, UK, 1998
Postdoctoral Fellowship, Children’s Hospital Boston, Harvard Medical School, Boston, 1998-2001
Research Interests
Dr. Luke is investigating the biochemical and biological role of serpins in the model system, C. elegans. A major focus is the role of one serpin gene in the hypoosmotic shock lethal (osl) phenotype. Additionally, the role of this serpin in cell death pathways is being investigated. Specific projects include investigating the interacting proteinase involved with this serpin using genetic and proteomic techniques, as well as other non-proteinase genes involved with this phenotype. These studies involve a wide range of proteomic, microarray, genetic, biochemical, and molecular biological techniques.
Selected Publications
Silverman GA, Whisstock JC, Askew DJ, Pak SC, Luke CJ, Cataltepe S, Irving JA, Bird PI. Human clade B serpins (ov-serpins) belong to a cohort of evolutionarily dispersed intracellular proteinase inhibitor clades that protect cells from promiscuous proteolysis. Cell Mol Life Sci, 61(3):301-325, 2004.
Cole E, Miller D, Rometo D, Çataltepe S, Pak SC, Askew DJ, Silverman GA, Luke CJ. Identification and activity of a lower eukaryotic serpin from cyanea capillata: Analysis of a jellyfish serpin, Jellypin. Biochemistry, 43(37):11750-11759, 2004.
Askew DJ, Askew YS, Kato Y, Luke CJ, Pak SC, Bromme D, Silverman GA. The amplified mouse squamous cell carcinoma antigen gene locus contains a serpin (Serpinb3b) that inhibits both papain-like cysteine and trypsin-like serine proteinases. Genomics, 84(1):166-175, 2004.
Luke CJ. Serpin production using rapid in vitro transcription/translation systems. Methods, 32(2):191-198, 2004.
Pak SC, Kumar V, Tsu C, Luke CJ, Askew YS, Askew DJ, Mills DR, Bromme D, Silverman GA. Characterization of serpins in the nematode C. elegans: Role of Srp-2 in postembryonic development. J Biol Chem, 279(15):15448-15459, 2004.
Luke CJ, Pak SC, Askew DJ, Askew YS, Smith JE, Silverman GA. Selective conservation of the RSL-encoding, proteinase inhibitory-type, clade L serpins in Caenorhabditis species. Front Biosci, 11:581-594, 2006.
Professional Affiliations
Biochemical Society (UK)
Society for General Microbiology (UK)
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